Biosynthesis, optimization of process parameters and antimicrobial activity of silver nanoparticles from Moringa oleifera leaf extract
1 Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, P.M.B 0182 Gombe State, Nigeria.
2 Department of Science Laboratory Technology, Federal Polytechnic, Kaltungo, Gombe, Nigeria.
Research Article
Comprehensive Research and Reviews in Chemistry and Pharmacy, 2022, 01(01), 001–011.
Article DOI: 10.57219/crrcp.2022.1.1.0001
Publication history:
Received on 10 July 2022; revised on 13 August 2022; accepted on 15 August 2022
Abstract:
The present study was explored for biosynthesis, optimization of process parameters and antimicrobial activity of silver nanoparticles from Moringa oleifera leaf extract. When factors affecting the synthesis were optimized, the optimum conditions were temperature of 600C at time t, of 20 min, pH of 8.5, silver nitrate (AgNO3) concentration of 2m M and 20 mL leaf extract. Formation of Ag NPs was primarily confirmed by change in color of reaction mixture from pale yellow to dark brown. UV-Vis spectrophotometer showed maximum absorbance peak at 400nm at different time intervals indicating formation of Ag NPs. The surface morphology of Ag NPs investigated using SEM was found to be spherical with homogeneous morphology. The average diameter of the primary particles was found to be 27 nm with a standard deviation and 4.85% as a coefficient of variation. The bio synthesized Ag NPs demonstrated effective antibacterial activity against both grams positive and gram negative pathogens investigated. It revealed the highest zone of inhibition at 16.2 mm for E. coli and 15.9 for S. typhimurium which were followed by S. aureus at 15.2 mm and B. cereus at 13.5 mm. On the other hand, the deionized water (negative control) and the leaf extract exhibited no any zone of inhibition. The AgNO3 being the positive control displayed antimicrobial efficacy against all tested pathogens, with their activities around 11.4 mm and 9.6 mm for gram negative bacteria, E. coli and S. typhimurium respectively. In the same vein, gram positive bacteria, S. aureus and B. cereus both have their zone of inhibition at 9.5 mm. With the foregoing, one may easily suggest that, Ag NPs fabricated from Moringa oleifera leaf extract be used in the treatment of diseases caused by the pathogens investigated in this research as it demonstrated high antibacterial potentials.
Keywords:
Biosynthesis; Optimization; Process Parameters; Antimicrobial Activity; Silver Nanoparticles; Moringa oleifera
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